Bioneer ExiProgen™
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ExiProgen™ is an instrument that automatically synthesizes protein from DNA. This product performs In-vitro transcription and translation automatically when loaded with DNA coding a protein and a protein synthesis kit. By purifying this protein through affinity chromatography method, more than 90% pure protein can be obtained within 6 hours and up to 16 species can be produced at one time. In addition, highly expressed affinity tagged proteins can be purified in cell lines with high purity.
Features and Benefits
Built-in protocols optimized for protein synthesis-purification and the extraction of a wide variety of Nucleic Acid Samples
ExiProgen™ has more than 900 built-in protocols. Each has been optimized for conditions from sample sources to target nucleic acids leading to a successful protein synthesis-purification. This user-friendly interface allows reproducible and reliable results. The instrument software is also able to be upgraded automatically through the network connection to maintain the system to up-to-date with the best performing protocols.
Cooling Unit
ExiProgen™ ensures sample integrity which allows target protein to maintain functionality by housing a cooling unit where the elution tube rack is located. This presents a solution for overnight running and provide confidence in your results.
ExiProgen™ Magnetic block & Heating Unit
Provides a Magnet & Heating block for protein or nucleic acid purification, which allows better extraction efficiency. Magnetic field is applied for pulling magnetic particles from sample wells for washing and eluting proteins or nucleic acids. Temperature of the heating block is tightly controlled, allowing it to generate reliable and reproducible results for protein synthesis. This also allows for evaporating away any residual ethanol after nucleic acid purification (Patent KR 10-1025135).
Contamination Shield
Contamination shield is designed to protect the assay from cross-contamination during instrument operation. Whenever the pipette tips are moving, the contamination shield is always co-located under the tips, therefore eliminating the possibility of cross-contamination which is necessary when working with multiple samples.
Easy to use LCD Touch screen
The 3.5" touch screen maximizes efficiency by offering an intuitive interface with simple push-button operation for processes such as selecting protocols and controlling the UV sterilization lamp.
UV sterilization lamp
ExiProgen™ is equipped with a powerful UV sterilization lamp that enables the user to sterilize the instrument chamber before and-or after experiment. This removes possible inter-assay cross-contamination that may occur.
Functions
| Feature | Benefit |
| Fast Protein production | From DNA to Pure protein in about 6 hours |
| DNA purification | A wide variety of gDNA kits available |
| RNA purification | A wide variety of RNA kits available |
| Affinity protein purification | Sample preparation for bio-defense detection of various bacteria and viruses |
Applications
Enzyme engineering, protein evolution, Synthetic biology, Bio-energy R&D, Protein drug R&D, expression screening, functional analysis, assay development and antigen production.
Specifications
| Dimensions | 320 mm(W) x 500 mm(H) x 535 mm(D) 12.6 in(W) x 19.7 in(H) x 21.1 in(D) |
| Weight | 27 kg(59.5 lbs) |
| Temperature range | 15 - 30 °C(59 - 86 °F) |
| Humidity range | 20 - 80%, no condensation |
| Operating system | Built-in |
| User interface | 320 x 240 touch screen graphic LCD |
| Input Voltage | 100 - 240 VAC |
| Frequency | 50 - 60 Hz |
| UV sterilization | 15 minutes cycle |
| Communications | TCP-IP |
| Heat block | 40 - 90 °C(104 - 194 °F) |
| Cooling block | 4 °C(39.2 °F) |
