0.25% Trypsin Solution



₦656,500.00

{"name":"Elabscience Biotechnology"}

Cell Culture

7364582735930


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0.25% Trypsin Solution

General Information

Product Introduction Trypsin is a serine hydrolase that can cut the base-side segment of the lysine and arginine residue in the polypeptide chain, hydrolyze the protein between the cells, and destroy the connection between the cells, so that the tissue or the adherent cells are dispersed into a single cell. The activity of the trypsin is related to the characteristics of the tissue or the cells, the concentration of the trypsin, the temperature and the time of action. At pH 8.0 and 37℃, the effect of the trypsin is the best. Therefore, the concentration of trypsin, the temperature and the time of the pancreatic enzyme should be grasped to avoid the excessive damage of the cells.
In general, the frequently-used working concentration of the trypsin is 0.25%, while the concentration of trypsin for the semi-adherent cells or the cells is 0.05%. Since EDTA is able to bind Ca2+ and Mg2+ to destroy the cell connection to promote the dissociation of the cells, a certain amount of EDTA is often added to the trypsin solution to enhance the dissociation effect.
Ingredient Statement

With: 0.2 g/L EDTA
          2.5 g/L Trypsin

Concentration 0.0025
Format Liquid
Bacterial Detection Negative
Fungal Detection Negative
Mycoplasmal Detection Negative
pH 7.2-7.4
Endotoxin Level <3 EU/mL
Storage This product can be stored at -5~-20℃ for 12 months with shading light.
Shipping Ice bag
Expiration Date 12 months


Notes

1. This product is for research use only.
2. It is necessary to pay attention to the aseptic operation and avoid the contamination.
3. It is not suitable for long time storage at room temperature or 2-8℃.
4. Thaw in 2-8℃ and mix fully before use. Avoid repeated freezing and thawing. It is recommended to aliquot the solution and store at -5~-20℃.
5. Because the sensitivity to trypsin of different tissues or cells are differently. The reaction time of trypsin should be determined according to the actual situation. Otherwise it will affect the the adhesion and growth of cells.

Shipment

Benefit from reliable delivery options

Every day, we ship our laboratory equipment throughout Nigeria and internationally. We work with trusted and experienced logistics and courier partners to ensure safe and timely delivery. Shipping costs and delivery times depend on the items ordered and the recipient’s location. Alternatively, you may arrange to collect your order in person by appointment. Below is an overview of our shipping options and policies.

Shipping within Nigeria

All goods are insured as standard up to a value of ₦ equivalent of €25,000. Shipping costs are calculated based on the weight, dimensions, value of the shipment, and delivery destination within Nigeria. For detailed shipping information, please contact us directly—we will be happy to assist.

International shipping

For accurate international shipping costs, we require details of the specific product(s) you wish to order.

Please contact us by telephone or email for a tailored quotation.

Freight forwarding, sea or air freight

For large, heavy, or specialized equipment, we provide personalised quotations for road freight, sea freight, or air freight. Taking current logistics conditions into account, we aim to offer the best possible price-to-performance ratio.

Incoterms 2020

All shipments are handled in accordance with Incoterms® 2020 (ICC). Please contact us to confirm and agree on your preferred delivery terms.

Self-collection

If required, you may collect your goods in person by prior appointment. Please note that pricing and availability may vary between Nigeria and other countries.

Standard deliveries

Standard shipments within Nigeria are handled by reputable courier and logistics providers. Upon request, we can also arrange alternative carriers to meet specific delivery requirements.

Insurance

All deliveries are insured up to a maximum value of ₦ equivalent of €25,000. If the value of your goods exceeds this amount, we will contact you to arrange additional insurance coverage.

Delivery time

Delivery times depend on product availability and order volume. High-demand items may be subject to delays beyond our control. If you require urgent delivery, please inform us in advance, and we will do our best to accommodate your request.

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0.25% Trypsin Solution
0.25% Trypsin Solution
₦656,500.00
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