CO2 Incubator

BINDER CO2 incubator CBF 260

The BINDER CO2 incubator CBF 260 is a high-performance device for cell cultivation and ideal for demanding applications in GMP environments. Thanks to its sophisticated design and precise regulation of temperature, humidity and CO2 value, it ensures optimum growth conditions for various cell types. Compared to other incubators, it impresses with its hot-sterilisation function and contamination-proof humidification system, which reduces maintenance and maximises reliability.

Properties and advantages

This incubator combines numerous advanced features that are specifically tailored to the requirements of biotechnological and pharmacological laboratories. The most important features include:

- Temperature range from +6 °C above room temperature to 50 °C for versatile incubation conditions
- High spatial temperature accuracy of ±0.4 K and temporal deviation of only ±0.1 K
- Humidity range between 50 and 95 % relative humidity, without conventional water tray
- IR CO2 sensor for precise monitoring of the CO2 content up to a maximum of 20% by volume
- Short recovery times after door openings (approx. 6 minutes for temperature, 5 minutes for CO2)
- Interior volume of 267 litres with seamless stainless steel inner chamber for easy cleaning
- Robust power of 1.5 kW at a nominal voltage of 200-230 V or 100-120 V

In addition, a CO2 fail-safe system and a fault-diagnostic controller with visual and audible alarm ensure a high level of protection for sensitive samples. Intuitive touchscreen operation and the option to read out measured values via USB support convenient device management. The option to use CO2 and O2 control flexibly makes it possible to precisely simulate hypoxic conditions.

Hot ice sterilisation at 180 °C and the fanless design reduce the risk of microbial contamination. As no water bath is required, the operating costs are very low. At the same time, the cleaning concept is particularly quick and easy to implement: After the sterilisation phase, the device can be put back into operation in compliance with standard protocols for sterile work. The advantages of this technology are particularly evident in the field of cell therapy, as even sensitive cell types can be cultivated in a stable and reproducible manner.

Certifications and standards

The incubator is designed for use in GMP environments and fulfils important safety and quality standards. Its design is based on recognised standards to demonstrate temperature distribution and operational stability. Thanks to the available accessories and extensive options, the system can also be easily customised to meet specific compliance guidelines.

Application examples

The versatile incubator covers a wide range of possible experiments. Common applications include:

- Cell biology research: long-term cultivation of mammalian cells and primary cultures under constant conditions
- Pharmaceutical and drug research: testing cell lines for reactions to new or existing active ingredients
- Tissue engineering: creation of tissue substitutes in laboratory environments, for example for skin, bone or cartilage tissue
- Biotechnological production processes: Fermentation and multiplication of cells in enzyme or antibody production

Culture media such as RPMI 1640, DMEM or MEM are often used in these processes, which are maintained at optimum quality thanks to precise humidity and CO2 control. If a regulated oxygen concentration is required, for example between 0.2 and 20 % O2, this can be realised via a corresponding option package. For cell therapy approaches, compartmentalisation can also be created to keep different cell lines separate.

FAQs

How often do I need to clean the interior?

Due to the hot sterilisation at 180 °C and the contamination-proof humidification system, the cleaning effort is significantly reduced. As a rule, routine cleaning is sufficient, depending on the frequency of use and laboratory standards.

Do I need to use a water bath?

No, the device has a direct humidification system without a water tray. This minimises the risk of contamination and significantly reduces maintenance work.

What happens in the event of a spontaneous CO2 shortfall or excess?

An integrated fail-safe mechanism automatically regulates the CO2 flow and ensures that the samples are not damaged by incorrect pH values. An alarm is also triggered in the event of deviations.

How quickly does the incubator recover after a door opening?

Both the temperature and CO2 level usually return to the set value within a few minutes (approx. 6 minutes for temperature, approx. 5 minutes for CO2) after a 30-second door opening, so that samples are optimally protected.

What options are available for O2 regulation?

In addition to the standard variant, versions with O2 control ranges from 0.2 to 20 % and even up to 10 to 90 % are available. This allows hypoxic or hyperoxic conditions to be simulated precisely.

Conclusion

The incubator is an excellent choice for modern laboratory environments thanks to its extensive range of functions, first-class temperature and humidity stability and efficient hot-ice sterilisation. Whether for cell culture work, drug research or demanding tissue engineering processes: It guarantees maximum contamination safety, user-friendliness and constant cultivation conditions. Its sophisticated system design and variability in CO2 and O2 control make it a reliable and flexible laboratory partner.

BINDER CO2 incubator CBF 170

Precise control of temperature, CO2 content and humidity is essential for cell culture work and microbiological applications. This device offers maximum contamination protection thanks to its sophisticated design and advanced features. It is characterised by economical operation and versatile application options, making it a convincing solution in demanding laboratory environments.

Detailed description

Thanks to hot-air sterilisation at 180 °C and the integrated anti-contamination concept, maximum safety is guaranteed for sensitive cell cultures. The system works without a water tray and instead humidifies via a contamination-protected system, which minimises the cleaning effort. An IR CO2 sensor ensures precise and reproducible CO2 regulation in the range from 0 to 20% by volume. The optionally available O2 control (0.2 to 20 vol.% or 10 to 90 vol.%) also makes the device suitable for hypoxic applications where precise oxygen conditioning is crucial.

The fanless interior concept with Venturi gas mixing nozzle enables a homogeneous distribution of CO2, which ensures constant growth conditions. An intuitive touchscreen controller supports the precise setting of all parameters. Data is logged automatically and can be read out and documented via USB. With this combination of precision, user-friendliness and freedom from contamination, the device fulfils the highest demands in GMP-compliant environments.

Main technical features (extract)

- Temperature range: +6 °C above room temperature to 50 °C, fast recovery time after door openings
- Humidity control: 50 to 95 % relative humidity, humidification-optimised interior design
- CO2 control: 0 to 20 % CO2 by volume, rapid stabilisation in just 5 minutes
- O2 option: 0.2 to 20 vol.% or 10 to 90 vol.% O2 for a wide range of cell culture requirements
- Internal volume: 170 litres with seamless stainless steel inner vessel and high resistance
- Hot-air sterilisation at 180 °C: reliable protection against contamination
- Data management: Ethernet and USB interfaces and internal data logger

In addition, the appliance scores points for its low operating noise of just 41 dB(A) and average energy consumption of 55 Wh-h. With its robust stainless steel housing and a net width of 680 mm, height of 870 mm and depth of 715 mm, it can be integrated into many laboratories to save space.

Certifications and standards

The CO2 incubator fulfils all current standards regarding electrical safety and EMC compatibility that are required in modern laboratories. Thanks to its GMP-compliant documentation options, it fits seamlessly into validated processes. Certifications such as CE and compliance with important hygiene and material selection standards emphasise the quality.

Application examples

- Cell culture research in biotechnology: optimal environment for mammalian cell lines, such as CHO cells or HEK293 cells
- Pharmaceutical research: In vitro tests for drug development, for example for screening active ingredients in multi-well plates
- Microbiological assays: precise cultivation of sensitive microorganisms
- Clinical laboratories: examination of tissue samples that require constant conditions for cell growth

The option of a hypoxic shelf life with adjustable O2 content makes the incubator particularly interesting for research projects in the field of cancer cells and tumour cell lines. Its high level of contamination protection also makes it ideal for GMP-compliant areas in which quality and hygiene standards have the highest priority in every handling process.

FAQs

How easy is it to clean

The appliance boasts a smooth, seamless inner bowl and does not have a water tray. This makes it particularly easy to clean. Hot air sterilisation at 180 °C also ensures contamination-free conditions all round.

Can the incubator be integrated into an existing GMP environment?

Yes, the extensive data export options and compatibility with common documentation requirements make the device very suitable for GMP applications.

What consumables are required?

The device does not require any permanent consumables as it is operated without a water tray and using a robust IR sensor. This contributes to low operating costs.

Can the device be set to more specific O2 values?

Yes, if you need O2 values below 20% or in the higher range up to 90%, you can select the corresponding model variant with O2 control and thus create optimum growth conditions for special cell cultures.

Conclusion

This CO2 incubator offers a high level of safety for sensitive cell and tissue cultures in laboratories that work in compliance with GMP and pay attention to optimised processes. With its hot-air sterilisation, fanless interior and precise humidity and gas control, it makes a decisive contribution to the quality and reproducibility of laboratory data. Its wide range of applications, reliable control technology and simple maintenance and documentation make it a reliable and economical solution for biotech, pharmaceutical and research laboratories.

BINDER CO2 incubator CB-S 260

The BINDER CO2 incubators from the CB-S series are specially designed for the safe and efficient cultivation of cell cultures. The model is ideal for routine laboratories and research facilities and impresses with its consistently high process reliability. The combination of 180 °C hot air sterilisation and a reliable infrared CO2 sensor guarantees optimum growth conditions and maximum freedom from contamination of sensitive samples. Thanks to its robust design and low maintenance requirements, this device proves to be an extremely economical solution that offers decisive advantages compared to alternative incubators.

Reliable temperature and humidity control

Cell cultures require precisely controlled environmental conditions in order to develop optimally. In the CO2 incubator, a fanless design and a PID controller ensure a uniform temperature distribution from +6 °C above room temperature to 50 °C. With a spatial deviation of just ±0.4 K at 37 °C and a temporal deviation of ±0.1 K, researchers and laboratory staff are on the safe side. The integrated humidification unit also provides effective protection against evaporation and offers a homogeneous climate with 90-95 % relative humidity.

The fast recovery time is particularly advantageous: after briefly opening the door (30 seconds), the interior only needs eight minutes to return to the original temperature of 37 °C. At a target concentration of 5% CO2 by volume, the gas level is even stable after just five minutes - an important point in order not to jeopardise sensitive cell lines.

Modern anti-contamination concept

The interior of the incubator is seamlessly made of stainless steel and has a discreet beaded structure as a slide-in support. This design is easy to clean and minimises possible deposits. In combination with the 180 °C hot air sterilisation and the reliable IR CO2 measurement technology, the risk of contamination is drastically reduced. The infrared sensor regulates the CO2 concentration drift-free and ensures exact values even over longer periods of use.

An integrated fault diagnosis system with visual and acoustic alarm serves as a further safety measure and provides immediate information if device parameters deviate. This enables a rapid response before cell cultures are damaged.

Central features briefly summarised

- Temperature: +6 °C above room temperature up to 50 °C
- Humidity range: 90...95 % r.h.
- CO2 range: 0...20 % by volume
- 180 °C hot air sterilisation and stainless steel interior
- Internal volume: 267 litres
- IR probe for precise CO2 measurement
- CO2 recovery time at 5% by volume after door opening: 5 minutes

In addition, this model can accommodate up to eight shelves. Each drawer can carry a maximum load of 10 kg, so that extensive sample series can be processed simultaneously. The outer door has a solid finish and keeps the temperature stable inside.

Easy operation and cost-effectiveness

One of the greatest advantages of this incubator is its ease of use. All settings are made using the easy-to-read LCD display, while the internal data recording can be read out via the USB interface. The Ethernet interface enables smooth integration into existing data networks. At the same time, the device operates energy-efficiently at 37 °C with a consumption of just 55 Wh-h. There are no consumables at all, which keeps operating costs low in the long term.

Relevant standards and certifications

In many laboratories, devices such as this CO2 incubator are indispensable for cultivating sensitive samples in accordance with applicable quality standards. The manufacturer produces in accordance with applicable European safety regulations (CE labelling). In addition, strict design and production specifications are met to ensure reliable laboratory results.

Concrete application examples

- Academic cell production: routine cultivation of various cell lines for research projects in molecular biology
. - Tissue culture: Optimal conditions for maintaining sensitive cell clusters, for example for the reconstruction of tissue samples.
- Pharmaceutical development: Efficient incubation for investigating cell interactions with active substances such as antibodies or recombinant proteins.
- Microbiological test series: Controlled CO2 atmosphere for bacterial or fungal cultures that are dependent on an increased CO2 concentration.

FAQs

How often should the interior be cleaned

Regular cleaning is advisable so that surfaces are wiped down and hot air sterilisation is carried out at least once a month to minimise the risk of contamination.

Can the device continue to be used during the sterilisation cycle?

No, the incubator is emptied for 180 °C hot air sterilisation, as the high temperatures would damage samples. Regular incubation operation then starts up again.

Which gas cylinders are recommended

Lab-quality CO2 gas should be used. It is important that the purity ensures a constant CO2 concentration in the interior when connecting, thus guaranteeing stable conditions.

What are the advantages of IR measurement technology?

The infrared sensor measures without drifting and responds quickly to changes in the CO2 level, keeping a precise CO2 concentration constant.

Conclusion

The CB-S 260 CO2 incubator combines reliability, user-friendliness and cost-effectiveness in one device. With its high temperature and CO2 precision, fanless interior and reliable hot air sterilisation, it is a powerful laboratory instrument that is particularly suitable for academic and research-related applications. With low maintenance and energy requirements, the incubator is a future-proof investment for demanding cell cultivation under strictly controlled conditions.

BINDER CO2 incubator CB-S 170

The BINDER CB-S 170 CO2 incubator is a specialised solution for precise cell cultivation and is particularly suitable for demanding applications in research laboratories and in biotechnology. The device provides reliable and constant conditions for optimised cell culture growth. Compared to similar products on the market, it impresses with efficient hot air sterilisation, a fanless interior and a sophisticated anti-contamination concept.

Advanced technologies for safe laboratory processes

This device is characterised by a range of intelligent functions to simplify work in research, development and production. With a hot sterilisation method at 180 °C, the system ensures unique safety and contamination protection. The stable pH environment is reliably maintained thanks to the drift-free infrared CO2 sensor, while an integrated Venturi CO2 gas mixing nozzle supports homogeneous CO2 distribution in the interior.

The inner chamber is made of seamless stainless steel, which makes it easy to clean. In addition, the fanless design eliminates unnecessary air currents, which in turn reduces soiling in the interior. An integrated PID controller with a clear LCD display facilitates operation, while a data logger outputs relevant measured values to a USB stick. In addition, troubleshooting is simplified by a diagnostic system with visual and audible alarms.

Technical highlights at a glance

The following are the key performance data that make the incubator a robust, versatile and economical solution:

• Temperature range: +6 °C above room temperature to 50 °C
• Recovery time after 30 s door opening at 37 °C: 6 minutes
• Humidity range: 90 to 95 % r.h.
• CO2 range: 0 to 20 % by volume
• IR CO2 sensor and Venturi gas mixing nozzle for pH-stable cultivation conditions
• Internal volume: 170 litres
• Sound pressure level: 41 dB(A)

The appliance is available in versions for 200-230 V and 100-120 V and fulfils the usual standards and directives, including CE marking. Thanks to a rated output of 1.3 kW, it is energy-efficient even in continuous operation; it also works particularly economically thanks to the customised heating and humidification system.

The interior allows for up to six shelves, which means that a large number of samples can be cultivated at the same time. The external dimensions (width 680 mm, height 870 mm, depth 715 mm) allow installation in many standard laboratory furniture. The high-quality workmanship ensures a long service life, while the stainless steel construction effectively reduces corrosion and contamination.

Examples of use

- Production and maintenance of a wide variety of cell lines in biotechnology laboratories
- Research on proteins, enzymes and antibodies in academia
- Pharmaceutical product development with a focus on in vitro drug testing
- Microbiological testing of yeasts and bacteria for diagnostics and quality control

FAQs

How does hot air sterilisation at 180 °C work?

In hot air sterilisation, the interior is heated to 180 °C. This temperature thoroughly disinfects all surfaces and minimises the risk of contamination for cultures.

Why is a fanless interior advantageous?

The fanless design prevents unwanted air turbulence and thus reduces the risk of particle carryover, providing optimal contamination protection for the samples.

How to control the CO2 content

The built-in infrared CO2 measuring system reacts drift-free and ensures constant CO2 concentrations. The desired value can be set and constantly monitored via the PID controller.

Which certifications are available

The device fulfils the current laboratory standards and has a CE mark. It therefore complies with European safety and quality guidelines.

How quickly does the CO2 concentration stabilise after door openings?

The incubator only needs around five minutes to restore the set CO2 level, even with short-term fluctuations caused by open doors.

Can the interior be flexibly customised for different areas of use?

Yes, several slide-in units can be used. Each drawer can hold up to 10 kg, which provides sufficient space for different sample containers.

Is it easy to operate

Thanks to the clear LCD display and logically structured menus, the system can be controlled intuitively. In addition, an audible and visual alarm informs you of any deviations.

Conclusion

The BINDER CB-S 170 CO2 incubator impresses as a reliable incubator for laboratory environments that place the highest demands on contamination protection, pH stability and user-friendliness. The combination of hot air sterilisation, durable stainless steel construction and innovative CO2 measuring system ensures optimum cell growth conditions. This makes it ideal for demanding research and routine applications in the biotech and pharmaceutical sectors. At the same time, the device impresses with its economical operation, clear control of all parameters and wide range of applications in everyday laboratory work.